The Wanderers boss feels the players will need to be at their best if they are to go through to the next round and warns that a number of factors will be different from what his players are used to - the crowd being one.
"It won't be easy as there won't be a lot of fans to watch this game. Our players are used to the big stage and atmosphere." Allardyce said
"But they will be left in no doubt how important to everyone it is to get a win. If the players don't have the right attitude they could be embarrased and that is the last thing we want" he added.
BOLTON SQUAD: Kevin Poole, Nicky Hunt, Bruno N'Gotty, Anthony Barness, Simon Charlton, Ibrahim Ba, Ivan Campo, Kevin Nolan, Henrik Pedersen, Kevin Davies, Mario Jardel, Jussi Jaaskelainen, Jeff Smith, Jonathan Walters, Charlie Comyn-Platt, Per Frandsen, Stelios Giannakopolous, Ricardo Gardner
GILLS SQUAD: Jason Brown, John Hills, Chris Hope, Barry Ashby, Ian Cox, Andy Hessenthaler, Paul Smith, Nicky Southall, Paul Shaw, Nayron Nosworthy, Rod Wallace, Leon Johnson, Tommy Johnson, Bertrand Bossu, David Perpetuini, Mamade Sidibe, Kevin James, Marlon King, Danny Spiller