The second string will play a number of games in the competition against their North West rivals after a revamp has lead to Wanderers entering the competition for the first time since the 1960's.
Glen Little, who has recently recovered from a hamstring injury will get a chance to start against the Red Devils in a bid to stake his claim for a first team place following his loan move from Lancashire rivals Burnley earlier in the month.
Charlie Comyn-Platt, a late substitute in last night's first team win over Walsall in the Carling Cup also starts as do fellow fringe players Jonathan Walters and Jermaine Johnson.
BOLTON RESERVES: Jeremy Bon, Ricky Shakes, Jason Talbot, Joey O'Brien, Charlie Comyn-Platt, Lewis Hamlin, Glen Little, Derek Niven, Jermaine Johnson, Jonathan Walters, Cleveland Taylor
SUBS: Duong Thach, Sam Ashton, Ricardo Vaz Te, Reece Jones, Reda Kribib, Michael Gillan
UNITED RESERVES: TBC
SUBS: