The game comes just two days after the sides faced each other in a thrilling 2-2 Premiership draw and reserve boss Neil McDonald will be able to call upon those who did not make the first team on Saturday including Michael Bridges and Blessing Kaku.
Frenchman Florent Laville is also expected to play some part in the game having come through last week's reserve team draw with West Bromwich Albion he will line up alongside Charlie Comyn-Platt in the heart of the defence.
United are expected to name a similar side which defeated Carlisle United with fringe first team players Kieran Richardson and Chris Eagles expected to be amongst the starters. Out of favour keeper Ricardo also played in that game and could once again be called upon for the trip to Leyland.
BOLTON RESERVES: Andy Oakes, Ricky Shakes, Jason Talbot, Florent Laville. Charlie Comyn-Platt, Blessing Kaku, Joey O'Brien, Reda Kribib, Dale Whitehead, Michael Bridges, Bedi Buval
SUBS: Ricardo Vaz Te, Bradley Hill, Chris Howarth, James Sinclair, Rhys Powell
UNITED RESERVES: Tom Heaton, Phil Picken, Paul Tierney, Mark Howard, Paul McShane, David Fox, Chris Eagles, David Jones, Sylvain Ebanks-Blake, Guiseppe Rossi, Bojan Djordic
SUBS: David Poole, Ben Collett, Crocket, Richard Jones, Floribert N'Galula
SUBS